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金鱼草花青素糖基转移酶基因的克隆与表达特性分析
Cloning and Expression Analysis of the AmGT1 Gene in Snapdragon
  
中文关键词:金鱼草  花青素  糖基转移酶基因  表达分析
英文关键词:Snapdragon  anthocyanin  glycosyltransferase gene  expression analysis
基金项目:中国烟草总公司贵州省公司项目(No. 201501)。
作者单位
燕一波 漳州城市职业学院园林园艺系 
郭玉双 贵州省烟草科学研究院/烟草行业分子遗传重点实验室 
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中文摘要:
      以白苏子(Perilla frutescens)花青素糖基转移酶基因为探针,通过电子克隆和RT-PCR的方法从金鱼草(Antirrhinum majus L.)叶片中克隆到花青素糖基转移酶基因(AmGT1)的全长cDNA,并对其表达特性进行分析。结果表明:AmGT1全长892 bp,编码277个氨基酸;进化分析表明AmGT1的氨基酸序列与白苏子的同源性最高为79%,与其他花青素糖基转移酶蛋白的同源性在42%~62%之间,表明AmGT1是从金鱼草中克隆的新的花青素糖基转移酶基因。实时定量RT-PCR分析表明:该基因在金鱼草叶片中的表达量最高,根中的表达量最低;该基因虽然在红色、粉色、黄色、白色花朵中均有表达,但在红色花朵中的表达量最高,且存在一个从紧蕾期到松蕾期的跃变。
英文摘要:
      The full-length cDNA of the anthocyanin glycosyltransferase gene(AmGT1)in snapdragon(Antirrhinum majus L.)was isolated through in silico cloning and RT-PCR confirmation based the anthocyanin glycosyltransferase gene of the common perilla(Perilla frutescens)as the probe. The sequence and expression characteristics were further analysed. The full-length of AmGT1 was 892 bp, encoding 277 amino acids. Phylogenetic analysis showed that the sequence identity between AmGT1 and Perilla frutescens was 79%, displaying the highest consistency and sequence identity with other found GT proteins was between 42%-62%. These results indicated that AmGT1 was a new-found plant glycosyltransferase gene. Quantitative RT-PCR analysis revealed that the expression level of AmGT1 was the highest in the leaf and lowest in the root; AmGT1 was expressed in red, pink, yellow and white flowers; but the expression level of red flowers was the highest and there was a jump from tight bud stage to loose bud stage.
燕一波,郭玉双.金鱼草花青素糖基转移酶基因的克隆与表达特性分析[J].热带作物学报,2017,38(6):1101~1105
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