巴西橡胶树  ; 法尼基焦磷酸合酶  ; 5＇调控序列  ; 顺式作用元件

法尼基焦磷酸合酶是异戊二烯生物合成途径中的一个关键酶，根据NCBI数据库中巴西橡胶树法尼基焦磷酸合酶基因HbFPS序列设计引物，克隆了HbFPS起始密码子上游1066 bp的5＇调控序列。序列分析表明，该序列A/T含量为77.39 %，含有典型的真核生物核心启动子区域，在该序列中发现了一些与真核生物典型的顺式调控元件相似的TATA-box、增强子元件、CAAT-box和GATA-box以及一些与激素、胁迫诱导、转录因子相关的顺式作用元件。构建了含有该序列的植物表达载体并转化烟草，对获得的转基因烟草T0代植

Hevea brasiliensis  ; farnesyl diphosphate synthase  ; 5' regulatory region  ; cis-regulatory element

Farnesyl diphosphate synthase is a key enzyme in isoprenoid biosynthesis. The 5' regulatory region of HbFPS from Hevea brasiliensis was cloned using GenomeWalker strategy. TATA box, CAAT box and other core configurations were found in this promoter region. Several sequences similar to eukaryotic cis-regulatory element were found in the 5'-UTR proximal 5' flanking sequence of HbFPS. A plant expression vector with 5' regulatory region of HbFPS from Hevea brasiliensis was constructed and transgenic tobacco plants were obtained by Agrobacterium-mediated transformation. GUS activity analysis revealed that the fragment of 5' regulatory region of HbFPS drived expression of the GUS gene transgenic tobacco plants.

基金项目: 国家自然科学基金(No.30960160)，农业部橡胶树生物学重点开放实验室/科技部-海南省热带作物栽培 生理重点开放实验室开放课题(No.KLOF0902)，海南省自然科学基金(No.809039，30822)；中央级公益性科研院所基本科研业务费专项(No.ITBBZD0711)