巴西橡胶树  ; 小橡胶粒子蛋白  ; 5′调控序列  ; 顺式作用元件

在橡胶树中小橡胶粒子蛋白是催化和调控天然橡胶生物合成的重要蛋白因子。根据小橡胶粒子蛋白基因(HbSRPP)的mRNA序列(GenBank登陆号：AF051317)设计引物，利用Genome Walking方法克隆了HbSRPP起始密码子上游1 159 bp的5′调控序列。序列分析表明，该序列A/T含量为68.25 %，含有典型的真核生物核心启动子区域(-141bp-99 bp),一些与真核生物典型的顺式调控元件相似的TATA-box、增强子元件、CAAT-box、GATA-box也存在序列中，此外还发现一

Hevea brasiliensis Willd. ex. Juss. Mull. Arg  ; small rubber particle protein  ; 5' regulatory region  ; cis-regulatory element

In rubber tree, small rubber particle protein (SRPP) is a key protein involved in rubber biosynthesis. The 5' regulatory region of HbSRPP from Hevea brasiliensis was cloned using GenomeWalker strategy. TATA box, CAAT box and other core configurations were found in this promoter region. Several sequences similar to eukaryotic cis-regulatory elements were found in the 5'-UTR proximal 5' flanking sequence of HbSRPP. The plant expression vector with　5' regulatory region of HbSRPP was constructed and transgenic tobacco plants were obtained by Agrobacterium-mediated transformation. GUS activity analysis revealed that the fragment of 5' regulatory region of HbSRPP driveed expression of the GUS gene transgenic tobacco plants.

基金项目: 国家自然科学基金(No.30960160)，国家天然橡胶产业技术体系辅助育种岗位专项资金项目(No.nycytx-34-GW1-2)。